Ohio Department of Agriculture
Animal Disease Diagnostic Laboratory
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LABORATORY NAME: Virology
Laboratory News & Information:
Diseases:
Contact this laboratory:
Ohio Department of Agriculture
Animal Disease Diagnostic Laboratory
8995 East Main Street
Reynoldsburg, OH 43068-3399
Phone: 614.728.6220
Fax: 614.728.6310
Email: virology@agri.ohio.gov
Mission:
The mission of the Virology Section is to protect and promote animal and human health by providing expedient and reliable diagnostic virology service to veterinarians, livestock owners, and state and federal regulatory agencies.
Services:
Major services provided by the Virology Section include detection and identification of viral pathogens and/or antibodies from animal tissues, serum, body fluids, and feces. The primary screening technique for the majority of necropsy specimens is direct immunofluorescence (FA) staining of frozen tissue sections. The Virology laboratory completes over three-thousand FA tests per year, with a turn-around time of less than one day per test. Direct and immuno-electron microscopy, hemagglutination, hemagglutination-inhibition, ELISA, and immunodiffusion are additional techniques used for virus identification. Our laboratory routinely maintains eight cell lines for virus isolation: Porcine kidney, Bovine turbinate, Swine testicular, Vero, Marc, Baby hamster kidney (BHK), Rabbit kidney (RK), and McCoy. Other cell lines are stored in an ultra-low freezer or liquid nitrogen and are available for use as needed. The laboratory also maintains embryonated eggs for avian virus isolation work. In addition to routine virus isolation from diagnostic specimens, we also culture semen for bovine viral diarrhea (BVD), infectious bovine rhinotracheitis (IBR) and chlamydia to meet export requirements, as well as serum to detect non-cytopathic BVD carrier animals. The Virology Section conducts a large number of serological tests on bovine and porcine samples.